Abstract
Chemerin is a recently discovered adipokine that participates in the regulation of many physiological and disorder-related processes in mammals, including metabolism, inflammatory reactions, obesity, and reproduction. We investigated how chemerin affects the transcriptome profile of porcine luteal cells. The luteal cells were acquired from mature gilts. After the in vitro culturing with and without chemerin, the total RNAs were isolated and high-throughput sequencing was performed. Obtained datasets were processed using bioinformatic tools. The study revealed 509 differentially expressed genes under the chemerin influence. Their products take part in many processes, important for the functions of the corpus luteum, such as steroids and prostaglandins synthesis, NF-κB and JAK/STAT signal transducing pathways, and apoptosis. The expression of the CASP3, HSD3B7, IL1B, and PTGS2 genes, due to their important role in the physiology of the corpus luteum, was validated using the quantitative real-time polymerase chain reaction (qPCR) method. The qPCR confirmed the changes of gene expression. Chemerin in physiological concentrations significantly affects the expression of many genes in luteal cells of pigs, which is likely to result in modification of physiological processes related to reproduction.
Highlights
Chemerin (CHEM) is an adipokine discovered more than 20 years ago, as a product of TIG2, later named as RARRES2
The aim of this study was to investigate the influence of CHEM on the transcriptomic profile of swine in vitro cultured luteal cells collected during the mid-luteal phase of the estrous cycle
56.63% of processed reads were mapped to coding DNA sequence (CDS) regions, 22.08% were aligned to untranslated regions (UTRs), 2.59% to introns, and 18.69% to intergenic locations (Figure 2)
Summary
Chemerin (CHEM) is an adipokine discovered more than 20 years ago, as a product of TIG2, later named as RARRES2. This substance was first identified during studies on the pathogenesis of psoriasis in 1997 as a product undergoing increased production under the influence of tazarotene [1]. The occurrence of RARRES2 mRNA was found, e.g., in the ovaries of women [2], mice [3], and rats [4]. In accordance to the Du and Leung studies, the swine CHEM amino acid sequence has the highest level of identity with respect to the human sequence (84%) among all of tested model animals – cattle, rats, and mice [5].
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