Abstract
It is shown that the virion-associated RNA polymerase of Phialophora virus A, a type 2 double-stranded RNA mycovirus with a genome consisting of three RNA species, is a transcriptase. Synthesis of single stranded RNA in vitro continues for a least 24 hours and after this time two full length transcripts are produced, on average, per dsRNA molecule, i.e.re-initiation of transcription occurs in this in vitro system. Analysis of the products by polyacrylamide gel electrophoresis indicated that the efficiences of transcription of all three double-stranded RNA species were similar. The transcriptase activity of Phialophora virus A differs from the replicase activity of Penicillium stoloniferum virus S, the only other type 2 double-stranded RNA mycovirus whose RNA polymerase has been characterised.
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