Abstract
Latex exploitation enhances latex regeneration in rubber trees. The latex exploitation-caused latex flow lasts from 10 min to a few hours, which is convenient for exploring the transcript profiling of latex metabolism-related genes at the different stages of latex flow. In the present study, the expression pattern of 62 latex metabolism-related genes involved in water transportation, carbohydrate metabolism, natural rubber biosynthesis, hormone signaling, ROS generation and scavenging, and latex coagulum across three stages of latex flow between rubber tree clones CATAS7-33-97 and CATAS8-79 were comparatively analyzed by quantitative real-time PCR. The two clones show differences in latex regeneration and have a different duration of latex flow. The results showed that the expression levels of 38 genes were significantly higher in CATAS8-79 latex than in CATAS7-33-97 during latex regeneration, while 45 genes had a notably higher expression level in CATAS8-79 latex during latex flow. Together with the activation of the MEP pathway and jasmonate pathway in CATAS8-79 latex, HbPIP1;3, HbPIP1;4, HbSUT3, HbSus3, HbHMGS1-2, HbMK should contribute to the high latex regeneration ability. The up-regulation of ethylene signaling and Hb44KD and the down-regulation of latex coagulation-related genes in CATAS8-79 latex might contribute to its longer latex flow duration. This study provides some cues for revealing the regulation of latex metabolism in rubber trees.
Highlights
MATERIALS AND METHODSNatural rubber is an essential industrial substance around the world
There was no significant difference in the rubber content of the latex of CATAS8-79 between at the early stage (1 min) and the middle stage (80 min), but there was at the late stage (150 min) when the rubber content showed a highly significantly decreased (P < 0.01) and was significantly lower (P < 0.05) than the rubber content in the latex of CATAS7-33-97 at the late stage of latex flow (Figure 1)
Studies show the expression level of HbAPX, HbSUT3 and HbNIN2 is positively related to its protein activity both in vivo and in vitro (Tang et al, 2010; Liu et al, 2015; Chao et al, 2015b)
Summary
MATERIALS AND METHODSNatural rubber (cis-1,4-polyisoprene) is an essential industrial substance around the world. 62 genes involved in water transport regulation, carbohydrate metabolism, rubber biosynthesis, jasmonate and ethylene signaling, ROS generation and scavenging and latex coagulation were analyzed by qRT-PCR between CATAS8-79 and CATAS7-33-97 during latex flow following tapping.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
