Abstract
The full complement of genes encoded by Orf virus (ORFV) is not yet known. A cDNA library was constructed using mRNA isolated 5 h post-infection from cells infected with ORFV in vitro and grown in the presence of cytosine arabinoside. Using 12 non-overlapping probes representing the entire genome of the Orf-11 strain of the virus, cDNA clones representing individual genes expressed early in infection were isolated. Thirty-eight early genes were identified, either via isolation of their cDNA from the library or via Northern blotting. Twenty-nine of the isolated cDNAs represented orthologues of other poxvirus genes or had been identified previously as genes of ORFV, whilst seven appeared unrelated to any known poxvirus gene or indeed to any known gene in the DNA databases. The sequences described in this paper constitute approximately 30 kb of the ORFV genome and contain the complete or partial sequence of 47 genes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
