Abstract
Comparative transcriptome analysis provides a useful tool for the exploration of plant–pathogen interaction by allowing in-depth comparison of gene expression between unaffected, inoculated and wounded organisms. Here we present the results of comparative transcriptome analysis in genetically identical one-year-old Scots pine ramets after wounding and inoculation with Heterobasidion annosum. We identified 230 genes that were more than 2-fold upregulated in inoculated samples (compared to controls) and 116 downregulated genes. Comparison of inoculated samp les with wounded samples identified 32 differentially expressed genes (30 were upregulated after inoculation). Several of the genes upregulated after inoculation are involved in protection from oxidative stress, while genes involved in photosynthesis, water transport and drought stress tolerance were downregulated. An NRT3 family protein was the most upregulated transcript in response to both inoculation and wounding, while a U-box domain-containing protein gene was the most upregulated gene comparing inoculation to wounding. The observed transcriptome dynamics suggest involvement of auxin, ethylene, jasmonate, gibberellin and reactive oxygen species pathways and cell wall modification regulation in response to H. annosum infection. The results are compared to methyl jasmonate induced transcriptome dynamics.
Highlights
IntroductionPathogen interaction by allowing in-depth comparison of gene expression between unaffected, inoculated and wounded organisms
Dainis Run, gis Genetic Resource Centre, Latvian State Forest Research Institute “Silava”, 111 Rigas st., LV-2169 Salaspils, Latvia; Abstract: Comparative transcriptome analysis provides a useful tool for the exploration of plant
Libraries obtained from control, wounded, and inoculated samples were mapped against an H. annosum reference transcriptome to confirm inoculation and to identify pathogen genes
Summary
Pathogen interaction by allowing in-depth comparison of gene expression between unaffected, inoculated and wounded organisms. We present the results of comparative transcriptome analysis in genetically identical one-year-old Scots pine ramets after wounding and inoculation with Heterobasidion annosum. We identified 230 genes that were more than 2-fold upregulated in inoculated samples (compared to controls) and 116 downregulated genes. Comparison of inoculated samp les with wounded samples identified 32 differentially expressed genes (30 were upregulated after inoculation). Massive parallel transcriptome sequencing analysis has the potential to deepen the pool of available information about differentially regulated genes and the likely biological effects of these changes. Some interesting data have been published identifying differentially expressed genes (DEGs) in white pine blister rust (caused by the biotrophic pathogen Cronartium ribicola) resistant genotypes of Pinus monticola [3]. Fusarium circinatum were detected using RNA-seq [4] and transcriptome-based research on the expression of pathogenesis-related genes of Pinus tecunumanii after inoculation with
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