Abstract
An in-situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes is presented. Exogenous RNA controls derived from yeast allow quantitative estimation of absolute endogenous transcript abundance
Highlights
One of the most tantalizing promises of gene-expression profiling technology has been to develop assays that measure expression of all genes in a given species [1]
Transcript selection is no longer restricted to library contents, allowing genes absent from National Institute on Aging (NIA) cDNA clone collections [11] to be included from other public sequence collections
The index has been the basis of gene/transcript identification and sequence selection for all oligonucleotide array designs subsequent to the NIA Mouse 22K Microarray v1.1
Summary
One of the most tantalizing promises of gene-expression profiling technology has been to develop assays that measure expression of all genes in a given species [1] This is especially important for the mouse, which is a standard model for various human diseases. The early and rapid development of murine bioinformatics resources such as the draft genome assembly [2] and numerous expressed sequence tag (EST) projects have bolstered the feasibility of developing such microarray platforms for the mouse. Because it has been difficult to identify all murine genes and correctly group genomic and expressed sequences into genes and transcripts, microarray platforms intended to cover all mouse genes are only being made widely available, long after the draft assembly was released. Platforms that utilize long oligonucleotides give high sensitivity, with the potential for transcript specificity sufficient to distinguish transcripts from the same locus or closely related gene-family members [4,5]
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