Transanethole protects against Global Cerebral Ischemia through its Anti-inflammatory and Anti apoptotic activity

Abstract

Aim and Objective: In this research study, transanethole at a dose of 250 and 500mg/kg p.o was investigated for its potency against Bilateral Common Carotid Artery occlusion (BCCAO) for 1 hr accompanied by 22 hrs reperfusion. Method: Healthy Albino Wistar rats (200–250gm) were divided randomly into 4 groups (n=9). Group I was considered as sham control, received 2% tween 80p.o, group II was considered as ischemic- reperfusion (disease control) and received 2% tween 80p.o and group III and IV were considered as prophylactic treatment groups and received transanethole at doses of 250mg/kg, p.o and 500mg/kg, p.o. respectively. After pretreatment with transanethole for14 days, rats were subjected to bilateral common carotid artery occlusion (BCCAO) for 1 hour accompanied by 22 hr reperfusion (I/R). After 22 hrs of reperfusion, motor coordination, behavioral assessment, infarct area, brain water content, excitatory mediators, inflammatory and apoptotic markers were determined. Results: Transanethole improved the motor coordination, lowered the brain water content, infarction volume and attenuated the levels of excitatory mediators. Western blotting analysis was performed to identify the proinflammatory mediators (TNF α, p38 MAPK), anti-inflammatory cytokine IL 10 and apoptotic marker proteins (Caspase 3, Bcl-2 and Bax) in brain tissue. Prophylactic treatment with trans anethole significantly down regulated the expressions of TNF-α, caspase 3, Bax, dephosphorylated p38 MAPK and upregulated the expressions of Bcl-2, IL 10 in a dose dependent manner in comparison with disease control group. Conclusion: An anti-inflammatory and anti- apoptotic activity of transanethole protected from cerebral ischemia reperfusion injury.