Abstract
In nematodes, a fraction of mRNAs contains a common 22 nucleotide 5′ terminal spliced leader (SL) sequence derived by trans splicing. Here, we show that a cell-free extract prepared from developing embryos of the parasitic nematode Ascaris lumbricoides catalyzes accurate and efficient SL addition to a synthetic pre-mRNA at an authentic trans splice acceptor site. SL addition occurs via a trans splicing reaction that proceeds through Y-branched intermediates. The branch-point is located at either of two adenosine residues located 18 and 19 nucleotides upstream of the splice acceptor site.
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