Abstract
IL‐15, crucial in maintaining memory CD8+ T cell development and homeostasis, mediates its functions mainly via a mechanism called trans‐presentation. Trans‐presentation is the phenomenon by which IL‐15Rα, the high affinity IL‐15 binding protein, presents IL‐15 to opposing cells expressing the IL‐15Rβ/γC signaling components. Our group has previously shown that memory CD8+T cell development requires IL‐ 15α expression by the hematopoietic cell compartment. However, the identity of the hematopoietic cell types mediating IL‐15 trans‐presentation to memory CD8+ T cells is presently unclear. We attempted to mirror homeostatic proliferation of memory CD8+ T cells in vitro, by co‐culturing memory CD8+ T cell lines expressinga single T cell receptor with syngeneic bone marrow (BM) derived APC, in the absence of foreign antigen. We used this model system to compare the capacity of different dendritic cells (DC) subtypes, monocytes, and macrophages obtained either from normal mice, or mice lacking IL‐15α, to trans‐present IL‐15. The results show different outcomes of IL‐15‐mediated proliferation, survival and memory markers expression on CD8+T cells, depending on the cell type and activation status of the IL‐15 trans‐presenter. The different capacity to trans‐present IL‐15 by various hematopoietic cells may help localize the niche in which CD8+T cell memory is maintained.
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