Abstract
Cellular contacts between HIV-1-infected donor cells and uninfected primary CD4(+) T lymphocytes lead to virus transfer into endosomes. Recent evidence suggests that HIV particles may fuse with endosomal membranes to initiate a productive infection. To explore the role of endocytosis in the entry and replication of HIV, we evaluated the infectivity of transferred HIV particles in a cell-to-cell culture model of virus transmission. Endocytosed virus led to productive infection of cells, except when cells were cultured in the presence of the anti-gp120 mAb IgGb12, an agent that blocks virus attachment to CD4, suggesting that endocytosed virus was recycled to the outer cell surface. Confocal microscopy confirmed the colocalization of internalized virus antigen and the endosomal marker dynamin. Additionally, virus transfer, fusion, or productive infection was not blocked by dynasore, dynamin-dependent endosome-scission inhibitor, at subtoxic concentrations, suggesting that the early capture of virus into intracellular compartments did not depend on endosomal maturation. Our results suggest that endocytosis is not a mechanism of infection of primary CD4 T cells, but may serve as a reservoir capable of inducing trans-infection of cells after the release of HIV particles to the extracellular environment.
Highlights
Endocytosis has been postulated as a route of HIV entry into cells
Virus transfer, fusion, or productive infection was not blocked by dynasore, dynamin-dependent endosome-scission inhibitor, at subtoxic concentrations, suggesting that the early capture of virus into intracellular compartments did not depend on endosomal maturation
Our results suggest that endocytosis is not a mechanism of infection of primary CD4 T cells, but may serve as a reservoir capable of inducing trans-infection of cells after the release of HIV particles to the extracellular environment
Summary
Endocytosis has been postulated as a route of HIV entry into cells. Results: Endocytosed virus led to productive infection of cells, except when cells were cultured with the anti-gp120 antibody IgGb12. Cell-to-cell transfer of HIV particles may be blocked by agents that prevent virus attachment, such as the anti-CD4 monoclonal antibody (mAb) Leu3a, the anti-gp120 mAb IgGb12, or the CD4-IgG2 fusion protein PRO542 [11], but is resistant to HIV entry inhibitors targeting virus coreceptors or gp41-dependent fusion [7, 12], suggesting that virus attachment to CD4 is the sole factor necessary to induce the uptake of HIV particles [13] and that virus capture may occur in the absence of virus fusion and the initiation of a productive infection. Trans-infection by Endocytosed HIV-1 ence of HIV attachment inhibitors such as the anti-gp120 mAb IgGb12, suggesting that endocytosed virus was recycled to the cell surface to initiate a productive virus infection
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