Abstract
Short chain fatty acids (SCFA) are the main end-products of anaerobic degradation of dietary fiber in the colon [1]. SCFA contribute immensely to the body’s energy requirement and regulate numerous cellular processes during both health and disease [2]. SCFA especially butyrate possess anti-cancer activity through induction of apoptosis and promotion of differentiation by modulating the transcription of a number of underlying genes [3]. The exact transport mechanism of SCFA remains ambiguously defined, Hence we aim here to characterize the mechanism of butyrate transport across both luminal and basolateral membranes of the colonic epithelial cells.
Highlights
Short chain fatty acids (SCFA) are the main end-products of anaerobic degradation of dietary fiber in the colon [1]
Our data indicate that butyrate transport in both Luminal membrane vesicles (LMV) and basolateral membrane vesicles (BLMV) is stimulated by pH and bicarbonate gradients
Butyrate uptake was significantly impaired by phloretin and 4-CHC, the classical inhibitors of monocarboxylate transporter, and not by SITS and DIDS
Summary
Luminal membrane vesicles (LMV) and basolateral membrane vesicles (BLMV) were isolated from the equine colon using cation precipitation and differential centrifugation techniques. Characterization and LMV and BLMV origin and purity were performed using immuno-blotting analysis and enzyme assays. The uptakes of [14C]-butyrate were measured by rapid stop filtration technique
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