Abstract
Trans vaccenic acid (TVA; trans-11 18:1) is a positional and geometric isomer of oleic acid and it is the predominant trans isomer found in ruminant fats. TVA can be converted into cis-9, trans-11 conjugated linoleic acid (c9, t11-CLA), a CLA isomer that has many beneficial effects, by stearoyl CoA desaturase 1 (SCD1) in the mammary gland. The health benefits associated with CLA are well documented, but it is unclear whether trans fatty acids (TFAs) from ruminant products have healthy effects. Therefore, the effects of TVA on the proliferation of MCF-7 human breast adenocarcinoma cells and MCF-10A human breast epithelial cells were investigated in the present study. Results showed that TVA inhibited the proliferation of MCF-7 cells but not MCF-10A cells by down-regulating the expression of Bcl-2 as well as procaspase-9. In addition, the suppressive effect of TVA was confirmed in SCD1-depleted MCF-7 cells. Our results suggested that TVA exerts a direct anti-carcinogenic effect on MCF-7 cells. These findings provided a better understanding of the research on the anti-carcinogenic effects of TVA and this may facilitate the manufacture of TVA/c9, t11-CLA fortified ruminant products.
Highlights
Trans fat is the common name for unsaturated fat containing trans-isomer fatty acids
The results of our study indicated that Trans vaccenic acid (TVA) at concentrations of 100 and 200 μM, similar to CLA, induces the down-regulation of Bcl-2 protein expression in MCF-7 cells (Figure 2a,b). c9, t11-CLA
All these results suggested that TVA may have a direct anti-carcinogenic effect on human mammary adenocarcinoma cells and the clinical application of TVA on cancer prevention can be expected
Summary
Trans fat is the common name for unsaturated fat containing trans-isomer fatty acids. Little is known about the health effects of ruminant trans fats [2]. Trans vaccenic acid (TVA, trans-11 18:1), a positional and geometric isomer of oleic acid which is predominantly found in ruminant-derived products. This isomer comprises about 60%–80% of the total natural ruminant trans fats [3]. CLA is predominantly found in food from ruminant products such as milk, beef, and lamb. This compound has a lot of positive activities including anti-diabetic, fat reducing, and anti-carcinogenic effects [3,4,5,6]. In this study the effects of TVA on the proliferation of MCF-7 human breast adenocarcinoma cells and MCF-10A human breast epithelial cells were investigated
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