Tranexamic acid suppresses the supernatant of mast cell-enhanced permeability of rat IgE across rat endothelial cells

Abstract

It is a well-known fact that IgE is a key substance which induces the IgE-mediated allergic reaction in the extravascular tissue. However, it remains to be elucidated how IgE in the circulating blood transfers to the site of allergic reaction in the extravascular tissue. In this paper, the rat IgE passage through cultured rat aortic endothelial cells was examined using a dual chamber system. The effect of tranexamic acid was investigated for the supernatant of cultured mast cell-enhanced permeability of rat IgE across rat aortic endothelial cell. The permeability constant (PC) was used to evaluate the degree of the IgE passage and effect of tranexamic acid for IgE passage. PC of IgE was (0.29 ±0.13) × 10-5cm/sec in serum free medium and it was (1.13± 0.06) × 10-5cm/sec in the supernatant of cultured mast cell. In addition, tranexamic acid (t-AMCHA) of 5 × 10-3M, 1 × 10-2M, 5 × 10-2M at the concentration in upper chamber suppressed significantly the enhanced IgE passage by the supernatant of cultured mast cell in lower chamber. It was suggested from the above-mentioned results that tranexamic acid (Transamin®) might be useful to control IgE-mediated allergic reaction due to the inhibition of IgE transfer to extravascular site from the circulating blood.