Abstract
Nongenetically restricted T cells may be important host effector cells in women with ovarian cancer receiving intraperitoneal (ip) IL-2 therapy. We developed an in vitro technique to produce murine lymphokine-activated killer T cells. Murine splenocytes were cultured in the presence of 1000 U/ml IL-2 for 10 to 15 days. Phenotypical analysis showed 95% of total cells to express the pan T phenotype Thy 1.2 and no NK cell phenotypes by Day 7 in culture. These cells were labeled with 51Cr and their trafficking pattern after ip administration into normal and M5067 tumor bearing mice was examined. Various organs and tissues were collected at different timepoints and monitored for radio-activity. Within 4 hr., about 60% of the counts were associated with the bowel, peritoneum, and omentum of both normal and tumor bearing mice. About 15% of counts were associated with the blood, lung, kidney, spleen, and liver of both normal and tumor bearing mice.
Highlights
Immunotherapy is a new experimental approach to treat patients with recurrent ovarian carcinoma [l-3]
Studies in women with ovarian carcinoma reveal that T cells predominate the peritoneal ascites and the cells infiltrating solid tumor nodules on the peritoneal serosa [4-61
Animal studies of the trafficking patterns of activated T cells in the pc could be important for understanding the mechanisms involved and improving the clinical trials with biological response modifiers in women with this disease
Summary
Immunotherapy is a new experimental approach to treat patients with recurrent ovarian carcinoma [l-3]. In vitro studies have revealed that upon treatment with 11-2,T cells from both the pc and infiltrating solid tumors undergo proliferation and activation ficking of leukocytes in the pc, Reynolds et al, [14] demonstrated that syngeneic NK cell and nonactivated T cells remained free in the pc of rats for up to 24 hr after ip injection. Stevenson et al [15] demonstrated that human peripheral blood monocytes, activated by 24-hr incubation in vitro with IFN-7, rapidly adhere to the serosal surface of the peritoneal cavity when injected ip into cancer patients. Conducted to examine the trafficking pattern of radiolabeled T-LAK cells in the pc of normal mice and mice bearing an ip tumor which serves as a model for ovarian cancer. The results of this study demonstrate that within 4 hr, the majority of the counts are associated with the bowel and remain localized in the pc of both normal and tumor bearing mice
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