TRAF Family Member-Associated NF-KB Activator (TANK) Gene Polymorphism in Chronic Periodontitis and Peri-implantitis Patients

Abstract

This study sought to determine TANK (rs1921310, rs3820998) gene polymorphism in patients suffering from chronic periodontitis and peri-implantitis among an Iranian population. In this cross-sectional study, 77 chronic periodontitis and 40 peri-implantitis patients, along with 89 periodontally healthy subjects, were evaluated. A 5 cc venous blood sample was obtained from subjects. DNA was extracted using Miller's salting-out method and polymorphisms were determined by the use of KBioscience's Competitive Allele-Specific PCR (CASP) genotyping system. Data were analyzed using SPSS version 11.5 software. Differences in allele and genotype frequencies among patients and controls were evaluated with Kruskal-Wallis test. P<0.05 was considered statistically significant. No significant differences were detected between understudy groups in distribution of CC and CA genotypes and alleles when evaluating rs3820998 polymorphism or in distribution of GA and AG genotypes and alleles when assessing rs1921310 polymorphism. Within the limitations of this study, it seems that these two polymorphisms do not play a significant role in pathogenesis of chronic periodontitis or peri-implantitis among the Iranian population. However, we recommend performing similar studies with larger sample sizes among the Iranian population and other communities.