Traditional Uighur Medicine Karapxa decoction, inhibits liver xanthine oxidase and reduces serum uric acid concentrations in hyperuricemic mice and scavenges free radicals in vitro.

Nurmuhammat Amat,Ranagul Aziz,Mihrigul Anaydulla,Nicholas Moore,Parida Hoxur,Anwar Umar,Anake Kijjoa,Guzalnur Imam,Halmurat Upur

doi:10.1186/s12906-015-0644-1

Nurmuhammat Amat, Ranagul Aziz + Show 7 more

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https://doi.org/10.1186/s12906-015-0644-1

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Abstract

BackgroundKarapxa decoction (KD) is a Traditional Uighur Medicine used for hepatitis, cholecystitis, gastralgia, oedema, gout and arthralgia. Because of its purported effect in gout, its effects were tested in hyperuricemic mice models induced by yeast extract paste or potassium oxonate, as well as its capacity to scavenge free radicals in vitro.MethodsHyperuricemia was induced in mice by yeast extract paste or potassium oxonate. KD was given orally for 14 days at 200, 400 and 800 mg/kg/day, with Allopurinol 10 mg/kg/day as positive control. Serum uric acid (UA), and liver xanthine oxidase activity (XO) were measured. Scavenging activity of KD on 1, 1-diphenyl-2-picrylhydrazyl radicals (DPP•), nitric oxide (•NO), superoxide (O2•-), efficiency against lipid peroxidation, and XO inhibition were determined in vitro.ResultsKD inhibited liver XO activity and reduced serum uric acid in hyperuricemic mice. KD also showed noticeable antioxidant activity, scavenging free radicals (DPP•, •NO and O2•-). It was effective against lipid peroxidation and inhibited XO in vitro.ConclusionsThis study supports the traditional use of Karapxa decoction to treat hyperuricemia and gout.

Highlights

Karapxa decoction (KD) is a Traditional Uighur Medicine used for hepatitis, cholecystitis, gastralgia, oedema, gout and arthralgia
The aim of the present study was to evaluate the effects of KD in vivo on reduction of serum uric acid level and xanthine oxidase (XO) activity in hyperuricemic mice and to measure XO inhibition and free radical scavenging activity in vitro
Potassium oxonate was purchased from Aldrich Inc. 2, 2-diphenyl-1-picrylhydrazyl (DPP), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, phenazine methosulfate (PMS), nitroblue tetrazolium (NBT), nicotinamide adenine dinucleotide (NADH), Ascorbic acid (AA) and thiobarbituric acid (TBA) were supplied by Sigma Co. (St Louis, USA)

Summary

Introduction

Karapxa decoction (KD) is a Traditional Uighur Medicine used for hepatitis, cholecystitis, gastralgia, oedema, gout and arthralgia. The Traditional Uighur Medicine (TUM) herbal formula, Karapxa decoction (KD), composed of seven herbal ingredients (Table 1), including seed, leaves or roots of celery, chicory, fennel and dodders, has long been used for gout and arthralgia in addition to other symptoms such as Hepatic coldness,adiposis hepatica, jaundice, hepatitis, cholecystitis, gastralgia [8]. Other components of KD have effects, such as the hepatoprotective effect of Cuscuta chinensis against liver toxicity of acetaminophen and other drugs [8,12,13] It is not clear whether KD can reduce serum uric acid levels in hyperuricemia models and inhibit XO activities. The aim of the present study was to evaluate the effects of KD in vivo on reduction of serum uric acid level and XO activity in hyperuricemic mice and to measure XO inhibition and free radical scavenging activity in vitro

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