Abstract
We used the fluorescence ubiquitination-based cell cycle indicator (FUCCI) to monitor cell cycle arrest after treatment of FUCCI-expressing HeLa cells (FUCCI-HeLa) with a traditional Chinese medicine (TCM) herbal mixture LQ, previously shown to have anti-tumor and anti-metastatic activity in mouse models. Paclitaxel was used as the positive control. In 2D monolayer culture, the untreated control had approximately 45% of the cells in S/G₂/M phase. In contrast, the LQ-treated cells (9 mg/ml) were mostly in the G₀/G₁ (>90%) after 72 hours. After treatment with paclitaxel (0.01 μm), for 72 hours, 95% of the cells were in S/G₂/M. In 2.5D Matrigel culture, the colonies in the untreated control group had 40% of the cells in S/G₂/M. LQ arrested the cells in G₀/G₁ after 72 hours. Paclitaxel arrested almost all the cells in S/G₂/M after 72 hours. In 3D Gelfoam culture, the untreated control culture had approximately 45% of cells in G₂/M. In contrast, the LQ-treated cells were mostly in G₀/G₁ phase (>80%) after 72 hours treatment. Paclitaxel resulted in 90% of the cells arrested in S/G₂/M after 72 hours. The present report suggests the non-toxic LQ has potential to maintain cancers in a quiescent state for long periods of time.
Highlights
Traditional Chinese medicine (TCM) composi tions usually comprise multiple herbs and many components may be are necessary for efficacy
Effect of LQ or paclitaxel on the cell-cycle phase distribution of fluorescence ubiquitination-based cell cycle indicator (FUCCI)-HeLa cells growing in 2D culture
After treatment with LQ (9 mg/ml) for 72 hours, the HeLa-FUCCI cells were almost all arrested in G0/G1
Summary
Traditional Chinese medicine (TCM) composi tions usually comprise multiple herbs and many components may be are necessary for efficacy. In 2D monolayer culture, the untreated control had approximately 45% of the cells in S/G2/M phase. Paclitaxel resulted in 90% of the cells arrested in S/G2/M after 72 hours. The present report uses FUCCI imaging to demon strate the effect of LQ on the cell cycle arrest of HeLa cells.
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