Traction force and its regulation during cytokinesis in Dictyostelium cells

Abstract

Cytokinesis is the final stage of cell division. Dictyostelium cells have multiple modes of cytokinesis, including cytokinesis A, B and C. Cytokinesis A is a conventional mode, which depends on myosin II in the contractile ring. Myosin II null cells divide depending on substratum-attachment (cytokinesis B) or in a multi-polar fashion independent of the cell cycle (cytokinesis C). We investigated the traction stress exerted by dividing cells in the three different modes using traction force microscopy. In all cases, the traction forces were directed inward from both poles. Interestingly, the traction stress of cytokinesis A was the smallest of the three modes. Latrunculin B, an inhibitor of actin polymerization, completely diminished the traction stress of dividing cells, but blebbistatin, an inhibitor of myosin II ATPase, increased the traction stress. Myosin II is proposed to contribute to the detachment of cell body from the substratum. When the cell-substratum attachment was artificially strengthened by a poly-lysine coating, wild type cells increased their traction stress in contrast to myosin II null and other cytokinesis-deficient mutant cells, which suggests that wild type cells may increase their own power to conduct their cytokinesis. The cytokinesis-deficient mutants frequently divided unequally, whereas wild type cells divided equally. A traction stress imbalance between two daughter halves was correlated with cytokinesis failure. We discuss the regulation of cell shape changes during cell division through mechanosensing.