Abstract
To increase the reproducibility of cell culture studies, parameters such as local oxygen concentration should be reported. Moreover, the oxygen information provided by continuous monitoring allows for more control and can be used to change the dynamics of cell culture systems rapidly, even alter stem cell fates. We have measured dissolved oxygen concentrations inside standard tissue culture plates containing hydrogel scaffolds with primary human intestinal epithelial stem cells or human umbilical vein endothelial cells. We also used a microphysiological system to allow for continuous low oxygen, or hypoxic, culture of human stem cells. We found that the local oxygen concentration at the cell-media interface was much lower than the oxygen available in normobaric incubators, and that the cells may adjust to local oxygen level by altering their metabolic consumption rates.
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