Abstract
In order to trace the in vivodynamics of cancer cells, we introduced jellyfish aequorin cDNA into metastatic (A-11) and nonmetastatic (3LL) Lewis lung cancer cell lines. The transformants ( e.g.,A-11-4 and 3LL-24) remained highly tumorigenic when they were s.c.injected into C57BL/6 mice and the A-11-4 clone produced metastatic tumors in the lung. Luminescence produced by the expressed aequorin was detectable in these transformants in culture as well as in cells of primary or secondary tumors. The limit of detection of the aequorin assay was ≈90 cells, whereas ≈5 ×10 4cells could be macroscopically seen as metastatic foci in the lung. Thus, the aequorin assay is ≈500-fold more sensitive than macroscopic examination in detecting cancer metastasis.
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