Abstract
Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1+ cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elusive. In the present study, we first assessed the cell fate of PRX1+ cells during molar development and periodontal ligament (PDL) formation in mice. Furthermore, single-cell RNA sequencing analysis was performed to study the distribution of PRX1+ cells in PDL cells. The behavior of PRX1+ cells during PDL reconstruction was investigated using an allogeneic transplanted tooth model. Although PRX1+ cells are spatial specific and can differentiate into almost all types of mesenchymal cells in first molars, their distribution in third molars is highly limited. The PDL formation is associated with a high number of PRX1+ cells; during transplanted teeth PDL reconstruction, PRX1+ cells from the recipient alveolar bone participate in angiogenesis as pericytes. Overall, PRX1+ cells are a key subtype of dental MSCs involved in the formation of mouse molar and PDL and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation.
Highlights
The cranial neural crest-derived mesenchymal stem cells (MSCs) regulate multiple events of the tooth development process.[1,2,3,4] a variety of MSC subtypes are involved in tooth development, the mechanism underlying their specific participation during molar development and remodeling of periodontal tissues needs to be investigated.[5]
A variety of MSC subtypes are involved in tooth development, the mechanism underlying their specific participation during molar development and remodeling of periodontal tissues needs to be investigated.[5]
We investigated the potential function of PRX1+ cells in molar development and periodontal ligament (PDL) tissue regeneration by exploring the fates of PRX1+ cells during molar development using a lineagetracing mouse model and single-cell sequencing
Summary
A variety of MSC subtypes are involved in tooth development, the mechanism underlying their specific participation during molar development and remodeling of periodontal tissues needs to be investigated.[5] Recently, genetic Cre-mediated lineage-tracing studies have revealed diverse populations of MSCs and differentiated functional cells.[6,7] It is believed that lineagetracing studies about MSC functions will open a new perspective to investigate tooth development and regeneration. The function of PRX1+ cells in tissue regeneration and repair could be related to angiogenesis.[20]
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