Tracheal Repair with Human Umbilical Cord Mesenchymal Stem Cells Differentiated in Chondrocytes Grown on an Acellular Amniotic Membrane: A Pre-Clinical Approach.

Paulo Ricardo Baggio Simeoni,Paulo André Bispo Machado Júnior,Dilcele Silva Moreira Dziedzic,Luiz Cesar Guarita-Souza,Julio Cesar Francisco,Lucia De Noronha,Katherine Athayde Teixeira De Carvalho,Nelson Bergonse Neto,Anna Flávia Ribeiro Dos Santos Miggiolaro,Nádia Nascimento Da Rosa,Guilherme Naves,Priscila E Ferreira Stricker,Rossana Baggio Simeoni,Meila Bastos De Almeida

doi:10.3390/life11090879

Paulo Ricardo Baggio Simeoni, Paulo André Bispo Machado Júnior + Show 12 more

Open Access

https://doi.org/10.3390/life11090879

Copy DOI

Abstract

Acellular amniotic membrane (AM) has been studied, with promising results on the reconstruction of lesioned tissues, and has become an attractive approach for tracheal repair. This study aimed to evaluate the repair of the trachea with human umbilical cord mesenchymal stem cells (hucMSCs) differentiated in chondrocytes, grown on an experimental model. Tracheal defects were induced by surgical tracheostomy in 30 New Zealand rabbits, and the acellular amniotic membrane, with or without cells, was covering the defect. The hucMSCs were isolated and cultivated with chondrogenic differentiation over the culture of 14 days, and then grown on the AM. In this study, the AM was biocompatible and hucMSCs differentiated into chondrocytes. Our results demonstrated an important role for AM with cultured cells in the promotion of immature collagen, known to produce tissue regeneration. In addition, cartilaginous tissue was found at the tracheal defects, demonstrated by immunohistology results. This study suggests that this biomaterial implantation can be an effective future therapeutic alternative for patients with tracheal injury.

Highlights

Stenosis of the trachea is a major cause of morbidity and mortality after endotracheal intubation or tracheostomy [1]
This study aimed to assess the potential improvement of trachea function using human umbilical cord mesenchymal stem cells (hucMSCs) differentiation on an acellular amniotic membrane for tissue repair in the tracheostomy model
All rabbits were housed under standard conditions with food and water ad libitum on a 12-h day/night cycle in individual cages, throughout the experimental period

Summary

Introduction

Stenosis of the trachea is a major cause of morbidity and mortality after endotracheal intubation or tracheostomy [1]. Several deleterious effects are known to occur on the tracheal mucosa, including inflammation during trauma, granulation tissue formation, fibrosis, or scar formation [2]. It is known that the trachea can be reduced by half in adults and to by a third in small children being the gold standard treatment the end-to-end primary surgical anastomosis. When the defect exceeds these limits, it has been tried to replace the trachea using organic and artificial tubes that causes severe complications such as infection, extrusion, obstruction, stenosis and chronic rejection [3]. There are many obstacles to overcome, such as the rapid degradation of growth factors [6]

Objectives

Methods

Results

Conclusion