Abstract
In this paper, sensitive detections on abrin are demonstrated in real-time by piezoresistive (PZR) cantilever sensors. With attempts to optimize the design and fabrication of the PZR cantilevers, the voltage fluctuation of the PZR sensors was decreased to 1μV and the signal to noise ratio (SNR) was increased significantly. By using the biotin–streptavidin functionalized cantilevers, sensitive detections on abrin were realized. A linear relation between the concentration of abrin and the output voltage of PZR sensors was established within the range of 0.08–40ng/mL (1.2–600pM) and a limit of detection (LOD) of 80pg/mL (1.2pM) was obtained in PBS buffer. Meanwhile, detections on orange juice and skim milk samples spiked with abrin were also performed, and the detection sensitivity decreased by 6–17% compared with the one in PBS buffer at the same concentrations. At last, a model of pseudo-first-order kinetics was used to analyze the interaction mechanism of abrin and biotinylated polyclone antibody (bio-PcAb) against abrin, which was consistent with the detection results. The proposed PZR cantilever sensors provide an economical platform for on-site detections of food contaminants with the advantages of high SNR, portability, and rapid response.
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