Abstract
Primary and secondary amines were isolated from whey as their 2,4-dinitrophenyl derivatives, separated by thin-layer partition chromatography, and identified by gas chromatography-mass spectrometry. Examination of raw and pasteurized sweet whey, reconstituted spray dried whey powder, and protein-free ultrafiltration permeate of sweet and acid wheys revealed the same qualitative distribution of amines. Amines identified were methyl-, ethyl-, isobutyl-, dimethyl-, isopropyl-, and n-amylamine, pyrrolidine, and piperidine.
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