Abstract
Antigens derived from freeze-thawed tachyzoites of Toxoplasma gondii are commonly used in commercial enzyme-linked immunosorbent assay (ELISA) systems for detection of toxoplasmosis. Antigen production from three sources (mice, cotton rats and tissue culture) was analysed quantitatively and quanlitatively, using Coomassie-stained sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) gels and Western blotting. The antigen extracts were tested with a murine FAST-ELISA system to ascertain their effectiveness in detecting antibodies against Toxoplasma. Cotton rats were found to provide the highest yield of parasites, with low host cell contamination. Antigen extracts prepared from cotton rat exudates were also found to give optimum results when tested in the FAST-ELISA system.
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