Abstract
During the 2010 cholera outbreak in Haiti, water and seafood samples were collected to detect Vibrio cholerae. The outbreak strain of toxigenic V. cholerae O1 serotype Ogawa was isolated from freshwater and seafood samples. The cholera toxin gene was detected in harbor water samples.
Highlights
During the 2010 cholera outbreak in Haiti, water and seafood samples were collected to detect Vibrio cholerae
At the Haiti National Public Health Laboratory (LNSP), bacteria were recovered from ultrafilters by back flushing with a surfactant solution, and the solution was added to an equal volume of 2× strength alkaline peptone water (APW)
If the APW culture was positive for the ctxA gene, isolates were obtained by streaking onto thiosulfate citrate bile salts (TCBS) agar as described for water samples
Summary
V. cholerae O1, serotype Ogawa, ctxA-positive strains were isolated from 2 irrigation canals north of Port-auPrince in Ouest Department (Table 1). Both of these canals were used for drinking water by the local population, and communities near the canals were heavily affected by the outbreak. The ctxAB and tcpA sequences differed by 1 nt polymorphism from prototypical classical and El Tor alleles, respectively. These isolates were recovered from 30-L freshwater samples having turbidities of 11 and 16 nephelometric turbidity units, which were among those with the lowest turbidity collected during this investigation. In addition to samples from which toxigenic V. cholerae was isolated, real-time PCR testing by FDA detected the ctxA gene in APW culture broths for 3 seawater samples and 3 other seafood samples
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