Toxicopathological Effect of Paraquat (PQ) and olive oil as antioxidant on Adult Albino Male Rats Epididymis

Abstract

Paraquat (PQ) is a commonly used herbicide that induces oxidative stress via reactive oxygen species (ROS) generatiaon. This study aimed to investigate the effects of the antioxidant (olive oil) against PQ induced oxidative stress in rats. Male rats (48) were randomly divided into 4 groups which administrated orally and daily for six weeks as follows: distilled water(C), 3mg PQ & 2ml Olive oil Kg B.wt(T1), 3mg/Kg B.wt PQ(T2) and 2ml/Kg B.wt Olive oil (T3)respectively. The animals scarified after 2, 4, 6 weeks to estimated the histopathological effect of paraqauat and olive oil as antioxidant on adult male Epididymis of rats There was a marked vacuolar degenerated deciliation of tubules with cellular debris containing immature rounded sperms.with thickening of interstitial stroma and fibrous thinking of capsule as well as Focal infiltration of inflammatory cells and some foreign body giant cells in the lumen of tubules was seen also focal reduction in epithelial cell heightother lesion showed severe distruction of epididymis structure. With tubuler atrophy &partial absent of sperms. Later increase the sperm amount inside the tubules and congested blood vessels thinking of capsule and interstitial stroma, Macrovacuolation, necrosis, infiltration of inflammatory cells mainly lymphocytes &neutrophilesin the lumen of tubules with cell debris and absent of sperms when examed at late stage of expirment. while the olive oil group showed no clear lesions. counclusion: there Paraquat effect on male reproductive system reveled from the histopathological changes of male reproductive organ (epididymis).