Toxicology study of Eucalyptus citriodora on the insect Doleschallia bisaltidae

Abstract

Toxicological Study of Eucalyptus citriodora on Doleschallia bisaltidae as a primary pest of Graptophyllum pictum, was carried out at the Entomology laboratory, ISMECRI, Bogor from January to March 2021. The research consisted of a toxicology study of E. citrodora and larva survival rate of D. bisaltidae. The toxicology study was carried out by direct spray to 3rd instar larva (contact) and spraying the leaves (residual). The study was designed in a completely randomized design with 3 replications and 6 treatments consisting of Eucalyptus oil concentration: (1) 20%; (2) 10%; (3) 5%; (4) 2.5%; (5) 1.25% and (6) control/water determined through a ranging test first. Contact application: thirty larva were sprayed with each treatment, then placed in a plastic box where ten larva were fed with fresh Graptophyllum leaves. Residual application: five treated Graptophyllum leaves were placed in a plastic box, then filled with ten larva each. Observations were carried out every day. The results showed that Eucalyptus LC50 value by contact was 0.0584 (Y = 8.39 + 2.75X), more toxic than that applied residually with an LC50 value of 3.9487 (Y = 4.51 + 0.81X). The survival rate of larva to imago was higher in the residual treatment compared to the contact.