Abstract
Background: Numerous studies have demonstrated that daphnetin (DAP) has anti-inflammatory, antioxidant, antibacterial, antitumor, anti-arthritic, and other extensive pharmacological effects. However, its genotoxicity has rarely been examined. Materials and Methods: The safety of DAP was evaluated by an acute toxicity test using a Salmonella typhimurium/mammalian microsomal enzyme assay (Ames test), bone marrow micronucleus test, acute skin allergy test, and local mucosal irritation test. Results: Mice received a dose of 100 mg/kg body weight through lavage, which is more than 175 times the whole-body effective dose and were continuously observed for 14 d; mice showed no signs of poisoning. In the Ames test, each dose of DAP resulted in numbers of revertant colonies that were 0.05) but was significantly different from that of the cyclophosphamide (CTX)-positive control group (P Abbreviations used: DAP: Daphnetin, CTX: Cyclophosphamide, DMSO: Dimethyl sulfoxide, CIA: Collagen-induced arthritis, PCB: Polychlorinated biphenyl, NS: Normal saline, PCE: Polychromatic erythrocyte, NCE: Normochromatic erythrocyte; LD50: The median lethal dose.
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