Abstract
Metal and metal oxide nanoparticles are being used in different industries now‐a‐days leading to their unavoidable exposure to humans and animals. In the present study, toxicological testing was done using nanoparticles of copper oxide, cerium oxide and their mixture (1:1 ratio) on zebra fish embryos and THP‐1 cell line. Zebrafish embryos were exposed to 0.01 μg/ml to 50 μg/ml concentrations of dispersed nanoparticles using a 96 well plate and their effects were studied at different hours post fertilization (hpf) i.e. 0 hpf, 24 hpf, 48 hpf, 72 hpf and 96 hpf respectively. Results showed that copper oxide nanoparticles has drastic effects on the morphology and physiology of zebra fish whereas cerium oxide nanoparticles and mixture of these nanoparticles did not show much of the effects. Comparable results were obtained from in vitro study using human monocyte cell line (THP‐1). It is concluded that these nanoparticles may cause toxicological effects to humans and environment.
Highlights
Nanoparticles (NP), with size ranging from 1 to 100 nm, are being manufactured and used in a wide range of applications like cosmetics, biomedicines, electronics, and environment remediation
This study is an attempt to access the cytotoxic potential of NP, namely copper oxide (CuO) NP and cerium oxide NP, and to study the combined effect of these NP on zebra fish embryos as well as THP1 cell lines
Concentrations of NP ranging from very low to high were used to access their toxic effects on zebra fish embryos and cell lines
Summary
Nanoparticles (NP), with size ranging from 1 to 100 nm, are being manufactured and used in a wide range of applications like cosmetics, biomedicines, electronics, and environment remediation This is because of their unique physicochemical properties such as quantum effects, surface area as well as unique electrical, mechanical, and imaging properties.[1,2]. Zebra fish embryos have transparent body which makes development of body organs easy to monitor Other features such as low cost, easiness to maintain and breed, wellcharacterized developmental stages, and availability of different transgenic lines increases its ability to access the toxicological effects of NP. Zebra fish embryos and THP 1 cell line were used to study the toxic effects of CuO NP, cerium oxide NP, and their mixture using 96-well plates, and various toxicological end points were considered. The results obtained from both in vitro and in vivo studies were compared, and their toxicity was evaluated
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