TOXICOLOGICAL EFFECT OF THE DISINFECTANT BIOCID-30 ON CHICK EMBRYO

Abstract

Two hundered and twenty chicken eggs were utilized in this experiment. The eggs were randomly divided into two main groups; the first dipped group includes 100 fertile eggs, which was subdivided into 5 subgroups, each of 20 fertile eggs. Two subgroups were dipped in Biocid-30 diluted 1:300 for one and 5 minutes respectively. Another two subgroups were dipped in Biocid-30 diluted 1:400 for one and 5 minutes respectively. The last subgroup was used as control and dipped for 5 minutes in distilled water. The second main inoculated group contains 120 fertile eggs and subdivided into three subgroups. The first one (50 eggs) inoculated intra air sac by Biocid-30 diluted (1:300), while the second subgroup (50 eggs) treated in the same manner with Biocid-30 diluted 1:400. The last 20 eggs were inoculated with sterile saline solution and considered as a control group. Treatments were done at the first day of incubation, which carried out at 37 C°. On 21st day of incubation, all eggs under study were examined and chicks were investigated for teratogenicity. The results showed marked decrease in hatchability, livability and chick size in all treated subgroups. Also there was a significant increase in dead and resorped chicks percents in all dilutions and periods. The teratological studies revealed a high percentage of skeletal malformations in all treatments especially in inoculation group which show incomplete ossification of frontal and carpus bones, beside absence of patella, curled caudal and pygostyle vertebrae. Concerning visceral abnormalities, the organs size was reduced especially liver with absence of nasal septum and conchae in addition to absence of palatine shelf.