Toxicological, Biochemical and Histopathological Evaluation of the Ethanolic extract of Seagrass-Enhalus acoroides in Albino wistar rats

Abstract

Abstract The purpose of this research was to examine the acute toxicity (14 days) and sub-acute toxicity (28 days) studies of ethanolic extract of Enhalus acoroides (EEEA) on male wistar albino rats. A single dose of 2000 mg/kg of the extract was administered to the male rats for acute study and for sub-acute study, dose levels of 100, 200 and 400 mg/kg/b.wt. of the extract was administered daily by oral gavage to the rats respectively. During the treatment periods, body weight, water and food consumption, clinical signs, mortality, biochemistry, hematology, organ weights, gross findings, and histopathology were examined. Imperative organs are subjected to histopathology. Upto 2 g/kg b.w. of EEEA did not show any mortality in the acute toxicity studies. Our results demonstrated that sub-acute treatment of the crude extract of Enhalus acoroides revealed there were no significant changes in the haematological and serum biochemical parameters seen in the treated groups compared to the control group. Histological studies revealed no abnormalities in liver, kidney and spleen tissue at the high dose level of EEEA. In conclusion, both the acute and sub-acute toxicity studies proved no clinical toxic symptoms and histopathological lesions, which show Ethanolic extract of Enhalus acoroides are safety at normal therapeutic doses.