Toxicological assessment of azomethine «C-18» and study of anthelmintic efficacy of its various doses in cattle affected with nematodirosis

Abstract

The parameters of acute toxicity of azomethine "C-18" and blood indicatorsin young cattle, naturally invaded by nematodiruses before and after treatment with this remedyand various anti-helmintic drugs, were studied. The therapeutic dose of azomethine in calvesaffected with nematodirosis was titrated. In the first experiment, the acute toxicity of azomethine "С-18" was studied in white outbred mice and rats. As the result of the studies, the average death dose of azomethine "С-18" was not established. The maximum dose administered was 2000 mg/kg. There was nolossin laboratory animals. In the second experiment, the effect of azomethine "С-18" on the blood indicators of helminth-infested cattle was investigated. It was established that in the blood of calves naturally infected with nematodiruses there were: erythropenia, hypohemoglobinemia, leukocytosis, eosinophilia, lymphocytopenia, giproteinemia, hypoglycemia, increased transaminase activity. After deworming these animals withtetramizole granulate, 10% albendazole suspension and azomethine "С-18" broken hematological parameters were completely restored up to physiological norm on the 30-th day. In the third experiment, the effect of different doses of azomethine "С-18" on the hematological indices of healthy cattle were studied. The animals were orally administered azomethine "С-18" in doses of 50.0 and 100.0 mg/kg. After that hypoglycemia, increased alkaline phosphatase activity and reduced urea content were found in serum ofcalves. In the fourth experiment, the anthelmintic efficacy of various doses of azomethine "С-18" in cattle affected with nematodirosiswas studied. It has been established that azomethine "С-18" in doses 2; 5; 10; 15 and 20 mg/kg showed approximately the sameintensity of efficacy, which ranged from 95.3 to 97.9%. The extensity of efficacywas 87.5%. The therapeutic dose was 2.0 mg/kg.