Abstract
A 6-h toxicity test of cosmetic ingredients (methylparaben, 2-phenoxyethanol, sodium dodecyl sulfate, triethanolamine) was developed, based on the photosynthetic maximum quantum yield (Fv/Fm) of immobilized gametophytes of the brown macroalga Undaria pinnatifida. From calculated EC50 values, the toxicity ranking of the tested ingredients is: SDS (0.0060%) > MP (0.0634%) > 2-PE (0.2418%) > TEA (3.7023%). Compared to the results from conventional endpoints with other ecotoxicity test organisms, measurements of Fv/Fm is a more sensitive indicator of the toxic effects of cosmetic ingredients. The present technique is simple, rapid, practical, accurate, and requires little space to carry out. This novel method will be a useful tool for assessing the toxicity of a wide range of cosmetic ingredients once the respective sensitivities are fully established.
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