Abstract
Background: One of requirements for a material to be used in dentistry is that the material must be non toxic and non irritant, as well as have biocompatibility properties, not triggering a detrimental effect on the biological environment, both locally and systemically. The first-line tests that must be conducted is in vitro tests, one of which is MTT Assay. Samples used in this research were fibroblast cells (cultured BHK-21 cells) since fibroblasts are the most important and largest components in pulp, periodontal ligament, and gingiva. Purpose: This research aimed to determine toxicity effect of taro leaf extracts (Colocasia esculenta L. Schoot) on BHK-21 fibroblast cells. Methods: This research is an experimental laboratory research using Post Test Only Control Group design. The research treatment are the administration of Taro leaf extract (Colocasia esculenta L. Schoot) with 100%, 80%, 60%, and 40% concentration on BHK-21 fibroblast cell. Results: The fibroblast cell life percentage in 100%, 80%, 60%, and 40% concentration respectively are 106,3%, 50,9%, 36,2%, and 30,1%. The toxicity results are obtained using MTT assay technique after 24 hours. The optical density absorbency values are read by ELISA reader and represent life cell viability. Conclusion: Taro leaf extract (Colocasia esculenta L. Schoot) at a concentration above 80% is considered as non-toxic material to BHK-21 fibroblast cells
Highlights
The oral cavity is the part of the body where the food enters
Microorganisms that are often found in dentures with acrylic material are Candida albicans.[3]
Samples used in this research were fibroblast cells (BHK 21 cell culture)
Summary
The oral cavity is the part of the body where the food enters. The oral cavity has several parts such as teeth, gingival sulcus, tongue, cheeks, and palate. Acrylic resin is the material most often used because it is cheaper and easier to apply.[2] Microorganisms that are often found in dentures with acrylic material are Candida albicans.[3] Candida albicans is one of the main organisms causing denture stomatitis because of its ability to attach and form biofilms in oral cavity tissue and denture surfaces and its resistance to antifungal agents. This biofilm can grow widely on removable denture acrylic resin material. Schoot) at a concentration above 80% is considered as non-toxic material to BHK-21 fibroblast cells
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