Toxicity Test of LD50 Zinc (II) N-Benzyl Methyl Dithiocarbamate Complex Compounds on White Mice (Mus muscles)

Abstract

Dithiocarbamate complex compounds can be used as an antibacterial and antifungal. Zinc compounds function as antioxidants, antibacterials, and zinc can stop the proliferation of cancer cells. Based on some of the activities of these groups, it is expected that the Zinc (II) N-Benzylmethyl Dithiocarbamate complex compound has the potential to be developed as a new drug candidate. A toxicity test must be carried out to determine the level of safety of the compound. The objective of this study was to determine the LD50 value and determine the category of toxicity of the Zinc (II) N-Benzyl methyl Dithiocarbamate compound in white mice (Mus muscles). Methods This research is a laboratory experimental study using 40 white mice, consisting of 20 male mice and 20 female mice. As for the treatment, namely the Na CMC group as a negative control, the dose of the test substance was 240, 480, 960mg/Kg BW. Observations of mice were carried out for 24 hours by observing the number of dead animals and seeing toxic symptoms, body weight, and OWR (Organ Weight Relative) then the data were analyzed statistically. Results The results showed that the dose of 960mg caused the most death with LD50 values of 954mg and 794 mg of toxic symptoms, weight loss in mice, and an effect on OWR.