Abstract
This study deals with the toxic effect of ionic liquid, 1-butyl-3-methylimidazolium bromide (BMImBr) on guppy fish, Poecilia reticulata. The fishes were exposed to various concentrations of ionic liquid for 96h. The activity of antioxidant enzymes viz. catalase, glutathione S-transferase and superoxide dismutase were found to be increased with increase in concentration. The BMImBr resistant bacterium were isolated from garden soil by enrichment method and identified as Rhodococcus hoagii VRT1 by 16S rDNA sequencing. An isolated bacterium was effective in biodegradation of compound in 8 days which was analyzed by changes in BOD and COD and later on confirmed by HRMS analysis. Higher concentrations of compound induced DNA damage in liver cells while degraded product did not show adverse impact on the DNA integrity.
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