Toxicity of leachate from smoked cigarette butts to terrestrial animals: A case study on the earthworm Eisenia fetida

Abstract

Cigarette butts, as easily overlooked littered wastes, have been evaluated for toxicity in various researches. In this study, we investigated the toxic effects of smoked cigarette butt leachate (SCBL) on the earthworm Eisenia fetida. The results showed the following: 1) E. fetida avoided SCBL in artificial soil, and the avoidance rate was positively correlated with the concentration of SCBL but negatively correlated with the exposure time; 2) the LD50 of SCBL on earthworms at 36 and 48 h of exposure were 3.71 × 10−4 and 2.67 × 10−4 butts/cm2, respectively. Moreover, both the body surface and intestinal tissues of E. fetida were damaged after exposure to SCBL; 3) the survival rates of E. fetida exposed to artificial soil with an SCBL of 3.6 butts/kg for 7 and 14 days were 80.00 ± 7.07 % and 68.00 ± 4.47 %, respectively; and 4) the mean biomass of the surviving E. fetida in all treated groups decreased with increasing SCBL concentration and exposure time. We concluded that SCBL exerted significant negative effects on soil animals, and suggested that SCBs should be collected, detoxified, and reused before entering the natural environment.