Toxicity of hydroxylated polychlorinated biphenyls (HO-PCBs) using the bioluminescent assay Microtox(®).

Abstract

Hydroxylated polychlorinated biphenyls (HO-PCBs) are toxic contaminants which are produced in the environment by biological or abiotic oxidation of PCBs. The toxicity of a suite of 23 mono-hydroxylated derivatives of PCBs and 12 parent PCBs was determined using the bacterial bioluminescent assay Microtox(®). All HO-PCBs tested exhibited higher toxicity than the corresponding parent PCB, with effect concentration 50% (EC50) ranging from 0.07 to 133mg L(-1). The highest toxicities were recorded with 4-hydroxylated derivatives of di-chlorinated biphenyls (EC50=0.07-0.36mg L(-1)) and 2-hydroxylated derivatives of tri-chlorinated biphenyls carrying a chlorine substituent on the phenolic ring (EC50=0.34-0.48mg L(-1)). The toxicity of HO-PCBs generally decreased when the degree of chlorination increased. Consistently with this observation, a significant positive correlation was measured between toxicity (measured by EC50) and octanol-water partition coefficient (pK ow) for the HO-PCBs under study (Pearson's correlation coefficient, r=0.74), which may be explained by the lower solubility and bioavailability generally associated with higher hydrophobicity. This study is the first one which assessed the toxicity of a suite of PCBs and HO-PCBs using the bioluminescent assay Microtox(®), showing an inverse correlation between toxicity and hydrophobicity.