Toxicity of glyphosate to animals: A meta-analytical approach

Abstract

Glyphosate (GLY)-based herbicides (GBHs) are the most commonly applied pesticide worldwide, and non-target organisms (e.g., animals) are now regularly exposed to GLY and GBHs due to the accumulation of these chemicals in many environments. Although GLY/GBH was previously considered to be non-toxic, growing evidence indicates that GLY/GBH negatively affects some animal taxa. However, there has been no systematic analysis quantifying its toxicity to animals. Therefore, we used a meta-analytical approach to determine whether there is a demonstrable effect of GLY/GBH toxicity across animals. We further addressed whether the effects of GLY/GBH vary due to (1) taxon (invertebrate vs. vertebrate), (2) habitat (aquatic vs. terrestrial), (3) type of biological response (behavior vs. physiology vs. survival), and (4) dosage or concentration of GLY/GBH. Using this approach, we also determined whether adjuvants (e.g., surfactants) in commercial formulations of GBHs increased toxicity for animals relative to exposure to GLY alone. We analyzed 1282 observations from 121 articles. We conclude that GLY is generally sub-lethally toxic for animals, particularly for animals in aquatic or marine habitats, and that toxicity did not exhibit dose-dependency. Yet, our analyses detected evidence for widespread publication bias so we encourage continued experimental investigations to better understand factors influencing GLY/GBH toxicity to animals.