Toxicity of Cypermethrin-chlorpyrifos and Butachlor to Tadpole of Quasipaa boulengeri

Abstract

The tadpole of Quasipaa boulangeri was taken as the research object in this paper. The toxic effects of cypermethrin-chlorpyrifos and butachlor on tadpole of Q.boulangeri were studied by acute toxicity experiment and subchronic toxicity experiment in order to master the toxicity of different types of pesticides on tadpole of Q.boulangeri and provide scientific basis for amphibian protection. The results of acute toxicity experiment were analyzed by the method of probability unit regression analysis. The results of the acute toxicity experiment were analyzed by means of the probability-unit regression analysis method, and the LC50 of cypermethrin-chlorpyrifos and butachlor to the 2-month-old frog tadpoles at 24h, 48h, 72h and 96h were 0.419, 0.357, 0.346L, 0.332 and 1.183, 1.108, 0.925 and 0.876(mg/L), respectively. In the subchronic toxicity experiment, we observed the accumulation effect of different concentrations of pesticides on tadpoles through the statistics of the growth and development of tadpoles exposed to the drugs for a long time. The results suggested that the body length and body weight of tadpoles increased slowly with the increase of time and concentration of pesticide in the solution during the experiment.