TOXICITY OF CARNOY’S SOLUTION—AN IN VITRO STUDY

Abstract

Objective: To compare the effect of 2 Carnoy’s solution formulations produced with substances from 2 different laboratories, evaluating the effects on human keratinocyte cell cultures. Study Design: Two different solutions were prepared, composed by reagents from distinct laboratories. Both solutions were applied on keratinocyte cell cultures in vitro. Different dilutions of the solutions were used. All the solutions were tested at 3 and 5 minutes. The cellular response was obtained through evaluation of the dead cells in the cultures at 3 different times. The purity analysis of all reagents was performed by high-performance liquid chromatography. Results: The national drug cytotoxicity in the concentration of 0.1%, after 3 and 5 minutes and after 24 hours was lower compared with the samples treated with imported reagents (P< .05). After 7 days, the cytotoxicity in the 0.5% concentration, regardless of exposure time, was lower in the national group. The amount of contaminating metals in the imported solutions was significantly lower than in the national solution. Conclusions: The Carnoy’s solution prepared with imported reagents showed to be purer compared with a solution prepared with national reagents, and this could have been reflected by the cytotoxic effect. Supported by FAPEMIG. Objective: To compare the effect of 2 Carnoy’s solution formulations produced with substances from 2 different laboratories, evaluating the effects on human keratinocyte cell cultures. Study Design: Two different solutions were prepared, composed by reagents from distinct laboratories. Both solutions were applied on keratinocyte cell cultures in vitro. Different dilutions of the solutions were used. All the solutions were tested at 3 and 5 minutes. The cellular response was obtained through evaluation of the dead cells in the cultures at 3 different times. The purity analysis of all reagents was performed by high-performance liquid chromatography. Results: The national drug cytotoxicity in the concentration of 0.1%, after 3 and 5 minutes and after 24 hours was lower compared with the samples treated with imported reagents (P< .05). After 7 days, the cytotoxicity in the 0.5% concentration, regardless of exposure time, was lower in the national group. The amount of contaminating metals in the imported solutions was significantly lower than in the national solution. Conclusions: The Carnoy’s solution prepared with imported reagents showed to be purer compared with a solution prepared with national reagents, and this could have been reflected by the cytotoxic effect. Supported by FAPEMIG.