Abstract
A total of 65 samples, consisting of 8 sample types, collected from the Jordan Valley, were examined for the presence of Bacillus thuringiensis and B. sphaericus and for their toxicity against the larvae of the fruit fly Drosophila melanogaster. The frequency of samples containing toxic aerobic spore-forming bacilli was 12%; of which 21.7% belonged to B. thuringiensis and 17.4% to B. sphaericus. The B. thuringiensis populations consisted of 5 serogroups: thuringiensis (H1), entomocidus (H6), pakistani (H13), autoagglunated, in addition to a new serotype. The B. sphaericus population consisted of 3 serogroups, and belonged to serovars H5, H9, and H13. All B. thuringiensis and B. sphaericus local isolates, in addition to the reference strains B. thuringiensis kuristaki, and B. thuringiensis israelensis, showed high toxicity towards 3(rd) instar larvae of D. melanogaster. The toxic concentrations ranged between 2.0 x 10(6) and 4.4 x 10(7) viable spores ml(-1).
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