Toxicity of Australian and French Polynesian strains of Gambierdiscus Toxicus (Dinophyceae) grown in culture: Characterization of a new type of maitotoxin

Abstract

Gambierdiscus toxicus strains isolated from Australia and French Polynesia were grown in modified f 2 and f 10 nutrient media and the cells extracted for ciguatoxin and maitotoxin. The high nutrient enrichment of f 2 media induced aberrant cell morphology in both strains whereas the majority of cells grown in f 10 media had the typical lenticular shape of wild G. toxicus cells. The Australian strain grew faster and produced greater cell densities than the French Polynesian strain. Different chromatographic types of maitotoxin were extracted from the two G. toxicus strains and purified to homogeneity using reverse-phase high-performance liquid chromatography. The toxins elicited similar bioassay signs in mice, but produced different death-time vs dose relationships. The maitotoxin extracted from the Australian strain eluted later on both straight-phase and reverse-phase chromatographic columns than did the maitotoxin extracted from the French Polynesian strain. The maitotoxin extracted from the French Polynesian strain was chromatographically identical to reference maitotoxin. For each strain no differences were found between maitotoxins extracted from cells grown in f 2 or f 10 media. Only one toxin was produced by each strain of G. toxicus. Ciguatoxin was not produced by either strain.