Abstract
was obtained from Wako Pure Co., 0saka, Japan. This compound was dissolved in dimethyl sulphoxide and then diluted in Eagle's minimum essential medium (Mochida et al. 1983). The human carcinoma cell line KB and the method of cultivation was as described (Mochida and Yamasaki 1984). Toxicity test methods used were as reported (Mochida and Yamasaki 1984). The ID50 values (50% inhibitory dose to growth of cells) was used as an index of the toxicity of the compound. Cultured human KB cells were fixed with glutaraldehyde and 0s04 and morphological changes in the cell surface were observed under a scanning electron microscope (SEM).
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