Toxicity, Biocompatibility, pH-Responsiveness and Methotrexate Release from PVA/Hyaluronic Acid Cryogels for Psoriasis Therapy.

Cătălina Cheaburu Yilmaz,Cornelia Vasile,Nela Bibire,Carmen-Lăcrămioara Zamfir,Raoul-Vasile Lupuşoru,Daniela Pamfil,Cătălina Lupușoru

doi:10.3390/polym9040123

Cătălina Cheaburu Yilmaz, Cornelia Vasile + Show 5 more

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https://doi.org/10.3390/polym9040123

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Abstract

Poly(vinyl alcohol)/hyaluronic acid cryogels loaded with methotrexate were studied. The physical–chemical characterization of cryogels was performed by FT-IR spectroscopy, scanning electron microscopy, differential scanning calorimetry and dynamic mechanical thermal analysis. Acute toxicity and haematological parameters were determined by “in vivo” tests. The biocompatibility tests proved that the obtained cryogels showed significantly decreased toxicity and are biocompatible. The pH-responsiveness of the swelling behaviour and of the methotrexate release from the poly(vinyl alcohol)/hyaluronic acid (PVA/HA) cryogels were studied in a pH interval of 2–7.4. A significant change in properties was found at pH 5.5 specific for treatment of affected skin in psoriasis disease.

Highlights

Biodegradable and biocompatible hydrogels used in drug delivery are obtained by both physical and chemical crosslinking strategies
Hydrogels of Poly(vinyl alcohol) (PVA) and Hyaluronic acid (HA) prepared by method elaborated by Fahmy et al [27] which was a modified as described in the procedure given in previous paper [25]
4 cm−1 ), differential scanning calorimetry (DSC) (Perkin Elmer Differential scanning calorimetry (DSC)-8000, Shelton, CT, USA within a temperature range of 30 and 220 ◦ C at a heat rate of 10 ◦ C/min under 20 mL/min nitrogen flow), and dynamic mechanical–thermal analysis (DMTA) (Anton Paar MCR301 Rheometer, Physica MCR, Berlin, Germany); polymer membranes under half-wet state with rectangular shape of 40 mm × 12 mm dimensions at a constant frequency of 1 Hz from −50 to 220 ◦ C temperature range) was performed according to the procedures and experimental details described in previous paper [25]

Summary

Introduction

Biodegradable and biocompatible hydrogels used in drug delivery are obtained by both physical and chemical crosslinking strategies. The physical and chemical properties of hydrogels can be controlled. By their relative deformability they can conform to the shape of the surface to which they are applied, and their muco- or bioadhesive properties are advantageous when used with intent to immobilize them at the site of application [1]. Before use in disease therapy, the obtained hydrogels are loaded with drugs Both quantity and homogeneity of drug loading depend on the morphology of hydrogels, swelling behaviour, and on drug solubility. The properties of each hydrogel should be tailored according to the desired drug delivery application

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