Toxicity beyond accumulation of Titanium after exposure of Mytilus galloprovincialis to spiked seawater

Abstract

Mytilus galloprovincialis was exposed to seawater spiked with 5, 50 and 100 μg L−1 of Titanium (Ti) for 14 days. Seawater was renewed after 96 h and new addition of Ti was done. A parallel experiment conducted in the absence of mussels showed that during the first 24 h after spiking, Ti concentrations in seawater rapidly decreased to values below 2 μg L−1. For this reason, along the entire experimental period (14 days) mussels were exposed to Ti during two short periods, in the beginning of the experiment and after seawater renewal. At 96 h, mussels exhibited low Ti concentrations (<2.5 μg g−1), close or not significantly different from a control condition (1.6 μg g−1 in the absence of Ti). Despite the low accumulated Ti in mussels’ tissues after both experimental periods (96 h and 14 days), biochemical markers indicated that mussels developed two main strategies: reduction of their metabolic capacity to avoid the uptake of Ti, and antioxidant and biotransformation defense mechanisms, such as the activation of SOD, CAT, GPx and GSTs enzymes that were triggered to prevent cellular damages. Nevertheless, oxidative stress occurred after 96 h or 14 days. The current study highlights that alterations of biological activity of M. galloprovincialis exposed to Ti goes beyond its accumulation in tissues.