Abstract
Extracellular fungal extract mediated silver nanoparticles were biosynthesized using the endophytic fungi Lasiodiplodia pseudotheobromae isolated from Eupatorium triplinerve. The ETAgNPs were characterized by UV-Visible, FT-IR spectroscopy, and SEM. The antimicrobial and toxicity effect of ETAgNPs was evaluated using Caenorhabditis elegans as an animal model. The life span assay and bacterial colonization assay using C. elegans confirmed the efficacy of the ETAgNPs against Staphylococcus aureus. The results revealed that the nanoparticles were nontoxic to C. elegans and decreased the mortality rate in C. elegans when fed with S. aureus. This is the first scientific report demonstrating the extracellular synthesis of ETAgNPs from L. pseudotheobromae and showed its efficacy in controlling S. aureus mediated mortality in C. elegans.
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