Toxicity assessment of poultry-waste biosynthesized nanosilver in Anabas testudineus (Bloch, 1792) for responsible and sustainable aquaculture development-A multi-biomarker approach

Abstract

The current study investigates the potential utilization of poultry intestines for the synthesis of stable silver nanoparticles (AgNPs) and their impact on fish physiology. The AgNPs were synthesized and characterized using various analytical techniques. The toxicity of AgNPs on Anabas testudineus was evaluated, determining a 96-h LC50 value of 25.46 mg l−1. Subsequently, fish were exposed to concentrations corresponding to 1/10th, 1/25th, 1/50th, and 1/100th of the estimated LC50 for a duration of 60 days in a sub-acute study. A comprehensive range of biomarkers, including haematological, serum, oxidative stress, and metabolizing markers, were analyzed to assess the physiological responses of the fish. Additionally, histopathological examinations were conducted, and the accumulation of silver in biomarker organs was measured. The results indicate that silver tends to bioaccumulate in all biomarker organs in a dose- and time-dependent manner, except for the muscle tissue, where accumulation initially increased and subsequently decreased, demonstrating the fish's inherent ability for natural attenuation. Analysis of physiological data and integrated biomarker responses reveal that concentrations of 1/10th, 1/25th, and 1/50th of the LC50 can induce stress in the fish, while exposure to 1/100th of the LC50 shows minimal to no stress response. Overall, this study provides valuable insights into the toxicity and physiological responses of fish exposed to poultry waste biosynthesized AgNPs, offering potential applications in aquaculture while harnessing their unique features.