Toxicity and Functional Tissue Responses of Two Freshwater Fish after Exposure to Polystyrene Microplastics.

Martha Kaloyianni,Dimitrios N Bikiaris,George Koumoundouros,Stavros Kalogiannis,Ioannis Sampsonidis,George Z Kyzas,Konstantinos Feidantsis,Despoina Xanthopoulou,Georgia Kastrinaki,Glykeria Malioufa,Dimitra A Lambropoulou,Dimitra C Bobori,Anastasia Dimitriadi

doi:10.3390/toxics9110289

Abstract

Microplastics (MPs)’ ingestion has been demonstrated in several aquatic organisms. This process may facilitate the hydrophobic waterborne pollutants or chemical additives transfer to biota. In the present study the suitability of a battery of biomarkers on oxidative stress, physiology, tissue function and metabolic profile was investigated for the early detection of adverse effects of 21-day exposure to polystyrene microplastics (PS-MPs, sized 5–12 μm) in the liver and gills of zebrafish Danio rerio and perch, Perca fluviatilis, both of which are freshwater fish species. An optical volume map representation of the zebrafish gill by Raman spectroscopy depicted 5 μm diameter PS-MP dispersed in the gill tissue. Concentrations of PS-MPs close to the EC50 of each fish affected fish physiology in all tissues studied. Increased levels of biomarkers of oxidative damage in exposed fish in relation to controls were observed, as well as activation of apoptosis and autophagy processes. Malondialdehyde (MDA), protein carbonyls and DNA damage responses differed with regard to the sensitivity of each tissue of each fish. In the toxicity cascade gills seemed to be more liable to respond to PS-MPs than liver for the majority of the parameters measured. DNA damage was the most susceptible biomarker exhibiting greater response in the liver of both species. The interaction between MPs and cellular components provoked metabolic alterations in the tissues studied, affecting mainly amino acids, nitrogen and energy metabolism. Toxicity was species and tissue specific, with specific biomarkers responding differently in gills and in liver. The fish species that seemed to be more susceptible to MPs at the conditions studied, was P. fluviatilis compared to D. rerio. The current findings add to a holistic approach for the identification of small sized PS-MPs’ biological effects in fish, thus aiming to provide evidence regarding PS-MPs’ environmental impact on wild fish populations and food safety and adequacy.

Highlights

Polystyrene is a thermoplastic synthetic polymer with appropriate thermal and mechanical properties and can be used in many applications even though it is characterised as a hard and brittle material
Both fish liver and gill samples exhibit similar control and exposed spectra (Figure 1, shown only spectra of zebrafish), depicting peaks corresponding to proteins; between 900 and 1300 cm−1 are phosphates mainly associated with RNA and DNA related nucleic acids, while in the 1300 and 1800 cm−1 region are protein (Amide I, II) bonds and in the 2700–3900 wavenumbers are peaks related to N–H stretching vibration of proteins [76]
Our results showed that exposure to polystyrene microplastics (PS-MPs) revealed a significant increase in carbonyl groups in liver and gills of both fish in relation to the respective controls (Figure 4)

Summary

Introduction

Polystyrene is a thermoplastic synthetic polymer with appropriate thermal and mechanical properties and can be used in many applications even though it is characterised as a hard and brittle material. The worldwide production of polystyrene was close to 15.61 million metric tons in 2019 and it is estimated to be stabilized at these levels for the years, while it covers about 5–6% of the global plastic production [1]. It is a colourless transparent polymer used in household applications, electronics, packaging, isolation foams, single used items like disposable cutlery, etc. In a recent literature search, only 16.2% of the published papers concerning microplastic pollution, were focused on freshwater environments [11]

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